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uc berkeley macrolab vector 2 bt  (Addgene inc)


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    Structured Review

    Addgene inc uc berkeley macrolab vector 2 bt
    Uc Berkeley Macrolab Vector 2 Bt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/uc+berkeley+macrolab+vectors+2+bt/pET+His6+TEV+LIC+cloning+vector+(2B-T)+(Plasmid+%2329666)/pm41093992-237-29-34
    Average 94 stars, based on 89 article reviews
    uc berkeley macrolab vector 2 bt - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: The SMC-family Wadjet complex protects bacteria from plasmid transformation by recognition and cleavage of closed-circular DNA.
    Article Snippet: .. For the expression and purification of proteins, jetA, jetB, jetC, and jetD genes from Pseudomonas aeruginosa PA14 were cloned into UC Berkeley Macrolab vectors 2-BT and 2-AT (Addgene #29666 and #29665, respectively) for N-terminal TEV protease-cleavable His6-tag and tagless constructs, respectively. ..

    Article Title: Architecture and activation mechanism of the bacterial PARIS defence system.
    Article Snippet: Bacteria and their viruses (bacteriophages or phages) are engaged in an intense evolutionary arms race.. While the mechanisms of many bacterial antiphage defence systems are known, how these systems avoid toxicity outside infection yet activate quickly after infection is less well understood.. Here we show that the bacterial phage anti-restriction-induced system (PARIS) operates as a toxin–antitoxin system, in which the antitoxin AriA sequesters and inactivates the toxin AriB until triggered by the T7 phage counterdefence protein Ocr.

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection
    Article Snippet: .. For the expression and purification of Goslar ChmA protein, the gene was cloned into UC Berkeley Macrolab vectors 2-BT (addgene #29666) for N-terminal TEV protease-cleavable His6-tag constructs. .. For protein expression, the construct was transformed into E. coli Rosetta2 pLysS (EMD Millipore), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection
    Article Snippet: .. For the expression and purification of Goslar ChmA protein, the gene was cloned into UC Berkeley Macrolab vectors 2-BT (addgene #29666) for N-terminal TEV protease-cleavable His6-tag constructs. .. For protein expression, the construct was transformed into E. coli Rosetta2 pLysS (EMD Millipore), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: Architecture and infection-sensing mechanism of the bacterial PARIS defense system
    Article Snippet: .. For protein expression, E. coli B185 AriA (NCBI Accession # WP_001007866.1), AriB (WP_000093097.1), and T7 Ocr (NCBI Accession # NP_041954.1) genes were cloned into UC Berkeley Macrolab vectors 2-BT (Addgene #29666) and 13S-A (Addgene #48323) for N-terminal TEV protease-cleavable His 6 -tag and tagless constructs, respectively. ..

    Article Title: Architecture and infection-sensing mechanism of the bacterial PARIS defense system
    Article Snippet: .. For protein expression, E. coli B185 AriA (NCBI Accession # WP_001007866.1), AriB (WP_000093097.1), and T7 Ocr (NCBI Accession # NP_041954.1 ) genes were cloned into UC Berkeley Macrolab vectors 2-BT (Addgene #29666) and 13S-A (Addgene #48323) for N-terminal TEV protease-cleavable His 6 -tag and tagless constructs, respectively. ..

    Purification:

    Article Title: The SMC-family Wadjet complex protects bacteria from plasmid transformation by recognition and cleavage of closed-circular DNA.
    Article Snippet: .. For the expression and purification of proteins, jetA, jetB, jetC, and jetD genes from Pseudomonas aeruginosa PA14 were cloned into UC Berkeley Macrolab vectors 2-BT and 2-AT (Addgene #29666 and #29665, respectively) for N-terminal TEV protease-cleavable His6-tag and tagless constructs, respectively. ..

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection
    Article Snippet: .. For the expression and purification of Goslar ChmA protein, the gene was cloned into UC Berkeley Macrolab vectors 2-BT (addgene #29666) for N-terminal TEV protease-cleavable His6-tag constructs. .. For protein expression, the construct was transformed into E. coli Rosetta2 pLysS (EMD Millipore), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection
    Article Snippet: .. For the expression and purification of Goslar ChmA protein, the gene was cloned into UC Berkeley Macrolab vectors 2-BT (addgene #29666) for N-terminal TEV protease-cleavable His6-tag constructs. .. For protein expression, the construct was transformed into E. coli Rosetta2 pLysS (EMD Millipore), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Clone Assay:

    Article Title: The SMC-family Wadjet complex protects bacteria from plasmid transformation by recognition and cleavage of closed-circular DNA.
    Article Snippet: .. For the expression and purification of proteins, jetA, jetB, jetC, and jetD genes from Pseudomonas aeruginosa PA14 were cloned into UC Berkeley Macrolab vectors 2-BT and 2-AT (Addgene #29666 and #29665, respectively) for N-terminal TEV protease-cleavable His6-tag and tagless constructs, respectively. ..

    Article Title: Architecture and activation mechanism of the bacterial PARIS defence system.
    Article Snippet: Bacteria and their viruses (bacteriophages or phages) are engaged in an intense evolutionary arms race.. While the mechanisms of many bacterial antiphage defence systems are known, how these systems avoid toxicity outside infection yet activate quickly after infection is less well understood.. Here we show that the bacterial phage anti-restriction-induced system (PARIS) operates as a toxin–antitoxin system, in which the antitoxin AriA sequesters and inactivates the toxin AriB until triggered by the T7 phage counterdefence protein Ocr.

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection
    Article Snippet: .. For the expression and purification of Goslar ChmA protein, the gene was cloned into UC Berkeley Macrolab vectors 2-BT (addgene #29666) for N-terminal TEV protease-cleavable His6-tag constructs. .. For protein expression, the construct was transformed into E. coli Rosetta2 pLysS (EMD Millipore), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection
    Article Snippet: .. For the expression and purification of Goslar ChmA protein, the gene was cloned into UC Berkeley Macrolab vectors 2-BT (addgene #29666) for N-terminal TEV protease-cleavable His6-tag constructs. .. For protein expression, the construct was transformed into E. coli Rosetta2 pLysS (EMD Millipore), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: Architecture and infection-sensing mechanism of the bacterial PARIS defense system
    Article Snippet: .. For protein expression, E. coli B185 AriA (NCBI Accession # WP_001007866.1), AriB (WP_000093097.1), and T7 Ocr (NCBI Accession # NP_041954.1) genes were cloned into UC Berkeley Macrolab vectors 2-BT (Addgene #29666) and 13S-A (Addgene #48323) for N-terminal TEV protease-cleavable His 6 -tag and tagless constructs, respectively. ..

    Article Title: Architecture and infection-sensing mechanism of the bacterial PARIS defense system
    Article Snippet: .. For protein expression, E. coli B185 AriA (NCBI Accession # WP_001007866.1), AriB (WP_000093097.1), and T7 Ocr (NCBI Accession # NP_041954.1 ) genes were cloned into UC Berkeley Macrolab vectors 2-BT (Addgene #29666) and 13S-A (Addgene #48323) for N-terminal TEV protease-cleavable His 6 -tag and tagless constructs, respectively. ..

    Construct:

    Article Title: The SMC-family Wadjet complex protects bacteria from plasmid transformation by recognition and cleavage of closed-circular DNA.
    Article Snippet: .. For the expression and purification of proteins, jetA, jetB, jetC, and jetD genes from Pseudomonas aeruginosa PA14 were cloned into UC Berkeley Macrolab vectors 2-BT and 2-AT (Addgene #29666 and #29665, respectively) for N-terminal TEV protease-cleavable His6-tag and tagless constructs, respectively. ..

    Article Title: Architecture and activation mechanism of the bacterial PARIS defence system.
    Article Snippet: Bacteria and their viruses (bacteriophages or phages) are engaged in an intense evolutionary arms race.. While the mechanisms of many bacterial antiphage defence systems are known, how these systems avoid toxicity outside infection yet activate quickly after infection is less well understood.. Here we show that the bacterial phage anti-restriction-induced system (PARIS) operates as a toxin–antitoxin system, in which the antitoxin AriA sequesters and inactivates the toxin AriB until triggered by the T7 phage counterdefence protein Ocr.

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection
    Article Snippet: .. For the expression and purification of Goslar ChmA protein, the gene was cloned into UC Berkeley Macrolab vectors 2-BT (addgene #29666) for N-terminal TEV protease-cleavable His6-tag constructs. .. For protein expression, the construct was transformed into E. coli Rosetta2 pLysS (EMD Millipore), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection
    Article Snippet: .. For the expression and purification of Goslar ChmA protein, the gene was cloned into UC Berkeley Macrolab vectors 2-BT (addgene #29666) for N-terminal TEV protease-cleavable His6-tag constructs. .. For protein expression, the construct was transformed into E. coli Rosetta2 pLysS (EMD Millipore), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: Architecture and infection-sensing mechanism of the bacterial PARIS defense system
    Article Snippet: .. For protein expression, E. coli B185 AriA (NCBI Accession # WP_001007866.1), AriB (WP_000093097.1), and T7 Ocr (NCBI Accession # NP_041954.1) genes were cloned into UC Berkeley Macrolab vectors 2-BT (Addgene #29666) and 13S-A (Addgene #48323) for N-terminal TEV protease-cleavable His 6 -tag and tagless constructs, respectively. ..

    Article Title: Architecture and infection-sensing mechanism of the bacterial PARIS defense system
    Article Snippet: .. For protein expression, E. coli B185 AriA (NCBI Accession # WP_001007866.1), AriB (WP_000093097.1), and T7 Ocr (NCBI Accession # NP_041954.1 ) genes were cloned into UC Berkeley Macrolab vectors 2-BT (Addgene #29666) and 13S-A (Addgene #48323) for N-terminal TEV protease-cleavable His 6 -tag and tagless constructs, respectively. ..

    Over Expression:

    Article Title: Identification of the bacteriophage nucleus protein interaction network.
    Article Snippet: .. For overexpression in E. coli, UC Berkeley MacroLab vectors 2-BT (ampicillin resistant, His6-TEV tag; Addgene, catalog number 29666) and 13-S (spectinomycin resistant, no tag; Addgene, catalog number 48323) were used. ..

    Article Title: Identification of the bacteriophage nucleus protein interaction network
    Article Snippet: .. For overexpression in E. coli , UC Berkeley MacroLab vectors 2-BT (ampicillin resistant, His -TEV tag; Addgene, catalog number 29666) and 13-S (spectinomycin resistant, no tag; Addgene, catalog number 48323) were used. ..



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